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Microarray gridding by Mathematical Morphology

Roberto Hirata Jr.Junior BarreraRonaldo F. HashimotoDaniel O. Dantas

DNA chips (microarray) biotechnology is a hybridization based process that makes possible to researchers to quantitatively analyze fluorescence signals that represent the relative abundance of mRNA from two distinct samples. The object of analysis is a pair of images that must be segmented, that is, we must find the subarray regions (set of groupped spots) and the positions of the spots in each subarray. In this paper, we introduce a new technique using morphological operators that performs automatic gridding procedures for subarray and spot segmentations. This technique has been implemented in a software and tested in a variety of microarray images with success.

http://sibgrapi.sid.inpe.br/rep-/sid.inpe.br/banon/2002/11.29.12.30

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